Dr. Abdoallah Sharaf

Job Position: Postdoc - Plant Epigenetics

Contact details

Phone: +420 387 775 935
E-mail: abdoallah.sharaf@umbr.cas.cz
Web: https://abdosha.wixsite.com/asharaf
Room: 104

Description

Research interests

High throughput sequencing, Epigenetic, non-coding RNAs, Evolution, Molecular genetics.

Recent publications

Sharaf A, De Michele R, Sharma A, Fakhari S, Oborník M (2019) Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in Chromera velia. Biomolecules 9, 647.
Sharaf A, Füssy Z, Tomčala A, Richtová J, Oborník M (2019) Isolation of Purified Plastids and Mitochondria from Chromera velia. PLANTA 1:1-11 https://doi.org/10.1007/s00425-019-03259-3
Sharaf A, Gruber A, Jiroutová K, Oborník M (2019) Characterization of Aminoacyl-tRNA synthetases in chromerids. Genes 10, 582 https://doi.org/10.3390/genes10080582
Sharaf A, Miroslav O, Hammad A, EL-AFIFI SI, Marei EM (2018) Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium Bacteriophages. PeerJ 6:e5687 https://doi.org/10.7717/peerj.5687
Sharaf A, Mercati F, Elmaghraby I, Elbaz RM, Marei EM (2017) Functional and Comparative Genome Analysis of Novel Virulent Actinophages Belonging to Streptomyces flavovirens. BMC Microbiology, 17(1):51.
Oriol Vidal, Cord Drögemüller, Gabriela Obexer-Ruff, Irene Reber, Jordi Jordana, Amparo Martínez, Valentin Adrian Bâlteanu, Juan Vicente Delgado, Shahin Eghbalsaied, Vincenzo Landi, Felix Goyache, Amadou Traoré, Michele Pazzola, Giuseppe Massimo Vacca, Bouabid Badaoui, Fabio Pilla, Mariasilvia D’Andrea, Isabel Álvarez, Juan Capote, Abdoallah Sharaf, Àgueda Pons, Marcel Amills (2017) Differential distribution of Y-chromosome haplotypes in Swiss and Southern European goat breeds. Scientific Reports 7, 16161. doi:10.1038/s41598-017-15593-1

Curriculum vitae

Education and Employment:

2019-present – Postdoctoral research: Iva Mozgova Laboratory , Institute of Plant Molecular Biology, Czech Academy of Science, University of South Bohemia, České Budějovice, Czech Republic.
2015-2019 – Postdoctoral research: Laboratory of Evolutionary Protistology, Institute of Parasitology, Czech Academy of Science, University of South Bohemia, České Budějovice, Czech Republic.
2014-2015 – Postdoctoral research: Institute of Biosciences and BioResources (IBBR), U.O.S. Palermo, Italy
2013-2014 – Postdoctoral research: Centre for Research in Agricultural Genomics (CRAG), University of Barcelona (UAB), Spain
2012-2013 – Postdoctoral research: Department of Genetic, Faculty of Vetinaria, University of Cordoba (UCO), Spain.
2003 – present Assistant Professor - Department of Genetic, Faculty of Agriculture, Ain Shams University (ASU), Cairo, Egypt.
2008-2012 – PhD Student: Department The Genetic, Faculty of Agriculture, Ain Shams University (ASU), Cairo, Egypt.

Scientific experiences:

Invited Speaker “Bacillus thuringiensis genome sequencing: from DNA extraction to annotation through NGS technology” International Center for Genetic Engineering and Biotechnology “ICGEB” course, 17-19 November 2018, CBS-Sfax, Tunisia.
Attended the "23rd International Bioinformatics workshop on Virus Evolution and Molecular Epidemiology (VEME)”, 26-31 August 2018, Robert Koch-Institut Berlin, Germany. https://rega.kuleuven.be/cev/veme-workshop/2018
Attended the "Methods of Functional genomics, EMBL workshop”, 17-19 May 2017, České Budějovice, Czech Republic.
4/2015 - Camel Genome Meeting" Food and Agriculture, Organization of the United Nations (FAO), Riyadh, KSA.
3/2015 - "Mouse Genetics; Models for Human Diseases" Course, International Center for Genetic Engineering and Biotechnology “ICGEB”, Trieste, Italy.
9/2014 - “the Automated Functional Annotation and Data Mining”, Valencia, Spain.

Books:

Abdoallah A. Sharaf, Molecular Genetic Characterization of Silybum Marianum, LAP LAMBERT Academic Publishing, ISBN-13: 978-3-659-50225-5, ISBN-10: 3659502251 and EAN: 9783659502255

Research projects:

The diversity of Polycomb Repressive Complex 2 (PRC2).
The Origin of exosymbionts.
Chromera Velia Response to Mercury and Cadmium- Stress at –omics levels (O. A. project 2016).

List of Publications:

Sharaf A, De Michele R, Sharma A, Fakhari S, Oborník M (2019) Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in Chromera velia. Biomolecules 9, 647.
Sharaf A, Füssy Z, Tomčala A, Richtová J, Oborník M (2019) Isolation of Purified Plastids and Mitochondria from Chromera velia. PLANTA 1:1-11 https://doi.org/10.1007/s00425-019-03259-3
Sharaf A, Gruber A, Jiroutová K, Oborník M (2019) Characterization of Aminoacyl-tRNA synthetases in chromerids. Genes 10, 582 https://doi.org/10.3390/genes10080582
Sharaf A., Miroslav O., Hammad A., S. I. EL-AFIFI and Marei E.M. (2018) Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium Bacteriophages. PeerJ 6:e5687 https://doi.org/10.7717/peerj.5687
Oriol Vidal, Cord Drögemüller, Gabriela Obexer-Ruff, Irene Reber, Jordi Jordana, Amparo Martínez, Valentin Adrian Bâlteanu, Juan Vicente Delgado, Shahin Eghbalsaied, Vincenzo Landi, Felix Goyache, Amadou Traoré, Michele Pazzola, Giuseppe Massimo Vacca, Bouabid Badaoui, Fabio Pilla, Mariasilvia D’Andrea, Isabel Álvarez, Juan Capote, Abdoallah Sharaf, Àgueda Pons, and Marcel Amills. (2017) Differential distribution of Y-chromosome haplotypes in Swiss and Southern European goat breeds. Scientific Reports 7, 16161 https://doi.org/10.1038/s41598-017-15593-1
Sharaf, A.; Mercati, F.; Elmaghraby, I.; Elbaz R.M. and Marei, E. M. (2017) Functional and Comparative Genome Analysis of Novel Virulent Actinophages Belonging to Streptomyces flavovirens. BMC Microbiology, 17(1):51.
Cherifi Youcef Amine, Gaouar Suheil Bechir Semir, Guastamacchia Rosangela, El - Bahrawy Khalid Ahmed, Abushady Asmaa Mohammed Aly, Sharaf Abdoallah, Harek Derradji, Lacalandra Giovanni Michele, Saïdi -Mehtar Nadhira, Ciani Elena (2017). Weak genetic structure in northern African dromedary camels reflects their unique evolutionary history. PLoS ONE 12(1):e0168672.
Ashraf Helmi and Abdoallah Sharaf (2017) Genetic differentiating Aphis fabae and Aphis craccivora (Hemiptera: Sternorranycha: Aphididae) populations in Egypt using mitochondrial COI. Biologia, 71(11):1266-1273.
Helmi Ashraf, Abdoallah, Sharaf and Heba, Ibrahim (2017) Fingerprinting of Leafhoppers on Medicinal and Aromatic Plants in Egypt Using ISSRs. Munis Entomology & Zoology, 112:224-233
Alex Clop, Abdoallah Sharaf, Anna Castelló, Sebastián Ramos-Onsins, Susanna Cirera, Anna Mercadé Sophia Derdak, Sergi Beltran, Abe Huisman, Merete Fredholm, Pieter van As and Armand Sánchez (2016) Identification of protein-damaging mutations in 10 swine taste receptors and 191 appetite-reward genes. BMC Genomics, 17:685.
Alex Clop, Abe Huisman, Pieter van As, Abdoallah Sharaf, Sophia Derdak and Armand Sanchez (2016) Identification of genetic variation in the swine toll‐like receptors and development of a porcine TLR genotyping array. Genetics Selection Evolution, 48:28.
Elmaghraby I., Francesco C., Sharaf A. , E. M. Marie , A. M. M. Hammad. (2015) Isolation and Identification of Bacillus megaterium Bacteriophages Via AFLP Technique. Current Research in Bacteriology Journal. 8 (4): 77-89.
Marei E.M., Elbaz R.M., Sharaf A.A. and El-afifi S.I. (2015) Isolation and Identification of a Temprate Phage Induced from Candida albicans. Journal of Virology Research, 4(1):039- 043.
Marei E.M., Elbaz R.M., Elmaghrabi I. and Sharaf A. (2014) Occurrence of Mulberry Mosaic Virus in Egypt. International Journal of Microbiology Research, 6(2):575-580.
Amal I. Khalil, Gazaa H. Morsy, Gehan H. Lashein, Dina I. Abd El- Mottaleb, Abdoallah A. Sharaf (2013) Molecular Variations Based on RAPD among Oxyurids in Egypt, Parasitology United Journal, 6(1): 39-50
Abdoallah A. Sharaf, Ahmed Bahieldin, Samir A. Ibrahim, A.Z. E. Abdelsalam and Ashraf A. Khalil (2010) Biochemical and Genetic Characterization of 12 Silybum marianum Accessions Collected from Borg El-Arab, an Egyptian Habitat, Functional Plant Science and Biotechnology, 5 (Special Issue 1): 22-29.

Teaching Introduction of Bioinformatics
Faculty of Agiculture, Ain Shams University (ASU), Egypt

Quantitative Genetics
Faculty of Agiculture, Ain Shams University (ASU), Egypt

Molecular Genetics
Faculty of Agiculture, Ain Shams University (ASU), Egypt

Publications

Total found: 9 records
Awad M., Sharaf A., Elrahman A., El-Saadany H.M., ElKraly O.A., Elnagdy S.M. (2021) The First Report for the Presence of Spiroplasma and Rickettsia in Red Palm Weevil Rhynchophorus ferrugineus (Coleoptera: Curculionidae) in Egypt Acta Parasitologica 66 : 593–604. DOI: 10.1007/s11686-020-00310-2
Haddad B., Gristina A.S., Mercati F., Saadi A.E., Aiter N., Martorana A., Sharaf A., Carimi F. (2020) Molecular Analysis of the Official Algerian Olive Collection Highlighted a Hotspot of Biodiversity in the Central Mediterranean Basin Genes 11 (3) : 303. DOI: 10.3390/genes11030303
Sharaf A., De Michele R., Sharma A., Fakhari S., Oborník M. (2019) Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in Chromera velia Biomolecules 9 : 647. DOI: 10.3390/biom9110647
Sharaf A., Füssy Z., Tomčala A., Richtová J., Oborník M. (2019) Isolation of plastids and mitochondria from Chromera velia Planta 250 : 1731–1741. DOI: 10.1007/s00425-019-03259-3
Sharaf A., Gruber A., Jiroutová K., Oborník M. (2019) Characterization of Aminoacyl-tRNA Synthetases in Chromerids Genes 10 : 582. DOI: 10.3390/genes10080582
Sharaf A., Oborník M., Hammad A., El-Afifi S., Marei E. (2018) Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium bacteriophages PeerJ 6 : e5687. DOI: 10.7717/peerj.5687
Sharaf A., Mercati F., Elmaghraby I., Elbaz R. M., Marei E. M. (2017) Functional and Comparative Genome Analysis of Novel Virulent Actinophages Belonging to Streptomyces flavovirens BMC Microbiology 17 : 51. DOI: 10.1186/s12866-017-0940-7
Vidal O., Drögemüller C., Obexer-Ruff G., Reber I., Jordana J., Martínez A., Bâlteanu V.A., Delgado J.V., Eghbalsaied S., Landi V., Goyache F., Traoré A., Pazzola M., Vacca G.M., Badaoui B., Pilla F., D’Andrea M., Álvarez I., Capote J., Sharaf A., Pons A., Amills M. (2017) Differential distribution of Y-chromosome haplotypes in Swiss and Southern European goat breeds Scientific Reports 7 : 16161. DOI: 10.1038/s41598-017-15593-1
Helmi A., Sharaf A. (2016) Genetically Differentiating Aphis fabae and Aphis craccivora Populations in Egypt Using Mitochondrial COI (Hemiptera: Sternorranycha: Aphididae) Biologia 71 : 1266-1273. DOI: 10.1515/biolog-2016-0151

Dr. Abdoallah Sharaf

Research interests

Recent publications

Sharaf A, De Michele R, Sharma A, Fakhari S, Oborník M (2019) Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in Chromera velia. Biomolecules 9, 647.

Sharaf A, Füssy Z, Tomčala A, Richtová J, Oborník M (2019) Isolation of Purified Plastids and Mitochondria from Chromera velia. PLANTA 1:1-11 https://doi.org/10.1007/s00425-019-03259-3

Sharaf A, Gruber A, Jiroutová K, Oborník M (2019) Characterization of Aminoacyl-tRNA synthetases in chromerids. Genes 10, 582 https://doi.org/10.3390/genes10080582

Sharaf A, Miroslav O, Hammad A, EL-AFIFI SI, Marei EM (2018) Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium Bacteriophages. PeerJ 6:e5687 https://doi.org/10.7717/peerj.5687

Sharaf A, Mercati F, Elmaghraby I, Elbaz RM, Marei EM (2017) Functional and Comparative Genome Analysis of Novel Virulent Actinophages Belonging to Streptomyces flavovirens. BMC Microbiology, 17(1):51.

Education and Employment:

2019-present – Postdoctoral research: Iva Mozgova Laboratory , Institute of Plant Molecular Biology, Czech Academy of Science, University of South Bohemia, České Budějovice, Czech Republic.

2015-2019 – Postdoctoral research: Laboratory of Evolutionary Protistology, Institute of Parasitology, Czech Academy of Science, University of South Bohemia, České Budějovice, Czech Republic.

2014-2015 – Postdoctoral research: Institute of Biosciences and BioResources (IBBR), U.O.S. Palermo, Italy

2013-2014 – Postdoctoral research: Centre for Research in Agricultural Genomics (CRAG), University of Barcelona (UAB), Spain

2012-2013 – Postdoctoral research: Department of Genetic, Faculty of Vetinaria, University of Cordoba (UCO), Spain.

2003 – present Assistant Professor - Department of Genetic, Faculty of Agriculture, Ain Shams University (ASU), Cairo, Egypt.

2008-2012 – PhD Student: Department The Genetic, Faculty of Agriculture, Ain Shams University (ASU), Cairo, Egypt.

Scientific experiences:

Invited Speaker “Bacillus thuringiensis genome sequencing: from DNA extraction to annotation through NGS technology” International Center for Genetic Engineering and Biotechnology “ICGEB” course, 17-19 November 2018, CBS-Sfax, Tunisia.

Attended the "23rd International Bioinformatics workshop on Virus Evolution and Molecular Epidemiology (VEME)”, 26-31 August 2018, Robert Koch-Institut Berlin, Germany. https://rega.kuleuven.be/cev/veme-workshop/2018

Attended the "Methods of Functional genomics, EMBL workshop”, 17-19 May 2017, České Budějovice, Czech Republic.

4/2015 - Camel Genome Meeting" Food and Agriculture, Organization of the United Nations (FAO), Riyadh, KSA.

3/2015 - "Mouse Genetics; Models for Human Diseases" Course, International Center for Genetic Engineering and Biotechnology “ICGEB”, Trieste, Italy.

9/2014 - “the Automated Functional Annotation and Data Mining”, Valencia, Spain.

Books:

Abdoallah A. Sharaf, Molecular Genetic Characterization of Silybum Marianum, LAP LAMBERT Academic Publishing, ISBN-13: 978-3-659-50225-5, ISBN-10: 3659502251 and EAN: 9783659502255

Research projects:

The diversity of Polycomb Repressive Complex 2 (PRC2).

The Origin of exosymbionts.

Chromera Velia Response to Mercury and Cadmium- Stress at –omics levels (O. A. project 2016).

List of Publications:

Sharaf A, De Michele R, Sharma A, Fakhari S, Oborník M (2019) Transcriptomic Analysis Reveals the Roles of Detoxification Systems in Response to Mercury in Chromera velia. Biomolecules 9, 647.

Sharaf A, Füssy Z, Tomčala A, Richtová J, Oborník M (2019) Isolation of Purified Plastids and Mitochondria from Chromera velia. PLANTA 1:1-11 https://doi.org/10.1007/s00425-019-03259-3

Sharaf A, Gruber A, Jiroutová K, Oborník M (2019) Characterization of Aminoacyl-tRNA synthetases in chromerids. Genes 10, 582 https://doi.org/10.3390/genes10080582

Sharaf A., Miroslav O., Hammad A., S. I. EL-AFIFI and Marei E.M. (2018) Characterization and comparative genomic analysis of virulent and temperate Bacillus megaterium Bacteriophages. PeerJ 6:e5687 https://doi.org/10.7717/peerj.5687

Sharaf, A.; Mercati, F.; Elmaghraby, I.; Elbaz R.M. and Marei, E. M. (2017) Functional and Comparative Genome Analysis of Novel Virulent Actinophages Belonging to Streptomyces flavovirens. BMC Microbiology, 17(1):51.

Ashraf Helmi and Abdoallah Sharaf (2017) Genetic differentiating Aphis fabae and Aphis craccivora (Hemiptera: Sternorranycha: Aphididae) populations in Egypt using mitochondrial COI. Biologia, 71(11):1266-1273.

Helmi Ashraf, Abdoallah, Sharaf and Heba, Ibrahim (2017) Fingerprinting of Leafhoppers on Medicinal and Aromatic Plants in Egypt Using ISSRs. Munis Entomology & Zoology, 112:224-233

Alex Clop, Abe Huisman, Pieter van As, Abdoallah Sharaf, Sophia Derdak and Armand Sanchez (2016) Identification of genetic variation in the swine toll‐like receptors and development of a porcine TLR genotyping array. Genetics Selection Evolution, 48:28.

Elmaghraby I., Francesco C., Sharaf A. , E. M. Marie , A. M. M. Hammad. (2015) Isolation and Identification of Bacillus megaterium Bacteriophages Via AFLP Technique. Current Research in Bacteriology Journal. 8 (4): 77-89.

Marei E.M., Elbaz R.M., Sharaf A.A. and El-afifi S.I. (2015) Isolation and Identification of a Temprate Phage Induced from Candida albicans. Journal of Virology Research, 4(1):039- 043.

Marei E.M., Elbaz R.M., Elmaghrabi I. and Sharaf A. (2014) Occurrence of Mulberry Mosaic Virus in Egypt. International Journal of Microbiology Research, 6(2):575-580.

Amal I. Khalil, Gazaa H. Morsy, Gehan H. Lashein, Dina I. Abd El- Mottaleb, Abdoallah A. Sharaf (2013) Molecular Variations Based on RAPD among Oxyurids in Egypt, Parasitology United Journal, 6(1): 39-50

Abdoallah A. Sharaf, Ahmed Bahieldin, Samir A. Ibrahim, A.Z. E. Abdelsalam and Ashraf A. Khalil (2010) Biochemical and Genetic Characterization of 12 Silybum marianum Accessions Collected from Borg El-Arab, an Egyptian Habitat, Functional Plant Science and Biotechnology, 5 (Special Issue 1): 22-29.

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